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mx3000® qpcr system  (Agilent technologies)


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    Structured Review

    Agilent technologies mx3000® qpcr system
    Mx3000® Qpcr System, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mx3000+qpcr+system/us12005094-233-7-10
    Average 90 stars, based on 1 article reviews
    mx3000® qpcr system - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Reverse Transcription:

    Article Title: Cooperative regulation of Zhx1 and hnRNPA1 drives the cardiac progenitor-specific transcriptional activation during cardiomyocyte differentiation
    Article Snippet: .. Total RNA was extracted from cells with TRIzol reagent (Invitrogen), and 500 ng RNA was reverse-transcribed with PrimeScript RT reagent kit (TaKaRa). qPCR was performed with SYBR green reagent (Bio-Rad) in the MX3000 qPCR system (Agilent). ..

    Real-time Polymerase Chain Reaction:

    Article Title: Cooperative regulation of Zhx1 and hnRNPA1 drives the cardiac progenitor-specific transcriptional activation during cardiomyocyte differentiation
    Article Snippet: .. Total RNA was extracted from cells with TRIzol reagent (Invitrogen), and 500 ng RNA was reverse-transcribed with PrimeScript RT reagent kit (TaKaRa). qPCR was performed with SYBR green reagent (Bio-Rad) in the MX3000 qPCR system (Agilent). ..

    Article Title: Interplay of starch debranching enzyme and its inhibitor is mediated by Redox-Activated SPL transcription factor
    Article Snippet: A total of 1 μg of extracted RNA was used in cDNA synthesis with AffinityScript QPCR cDNA Synthesis kit (Agilent technology, Canada). .. The qRT-PCR was carried out in the Mx3000 qPCR system (Stratagene, USA). ..

    Article Title: Differential expression and global analysis of miR156/ SQUAMOSA promoter binding-like proteins (SPL) module in oat
    Article Snippet: .. Optical strip tubes were used to perform qRT-PCR analysis using the Mx3000 qPCR system (Stratagene, USA) using 1 μl diluted cDNA, 10 μM gene-specific primers, and 10 μl Brilliant III Ultra-Fast SYBR® Green QPCR Master Mix (Agilent, USA). ..

    Article Title: Microsporidia in Commercially Harvested Marine Fish: A Potential Health Risk for Consumers
    Article Snippet: .. Amplification was performed in an Mx3000 qPCR System (Agilent, Santa Clara, CA, USA) with the following cycling conditions: 50 °C for 2 min, 95 °C for 15 min, and 40 cycles of 95 °C for 10 s, 60 °C for 20 s and 72 °C for 20 s. The dissociation curve begins with a 1 min incubation at 95 °C to melt the DNA and then a 30-s incubation at 55 °C, followed by a ramp up to 95 °C with Allpoints data collection performed during the ramp. ..

    Article Title: Microsporidia in Commercially Harvested Marine Fish: A Potential Health Risk for Consumers.
    Article Snippet: .. Amplification was performed in an Mx3000 qPCR System (Agilent, Santa Clara, CA, USA) with the following cycling conditions: 50 ◦C for 2 min, 95 ◦C for 15 min, and 40 cycles of 95 ◦C for 10 s, 60 ◦C for 20 s and 72 ◦C for 20 s. The dissociation curve begins with a 1 min incubation at 95 ◦C to melt the DNA and then a 30-s incubation at 55 ◦C, followed by a ramp up to 95 ◦C with Allpoints data collection performed during the ramp. ..

    Article Title: Transcriptional Analysis of the Pre-Erythrocytic Stages of the Rodent Malaria Parasite, Plasmodium yoelii
    Article Snippet: .. The Brilliant® II SYBR® Green QRT-PCR AffinityScriptTM Master Mix, 2-Step kit (Stratagene), was used to produce cDNA from 24hrLS, 48hrLS, RAS and wtSPZ total RNA derived aRNA, cDNA was diluted 1∶5 with water and 2 ul diluted cDNA was used in 12 ul reactions qPCR reactions performed on a Mx3000 qPCR system (Stratagene). qPCR conditions were as follows, 94°C for minutes followed by 40 cycles of 94°C secs, 55°C 15 secs, 72°C 30 secs, followed by dissociation curve. ..

    SYBR Green Assay:

    Article Title: Cooperative regulation of Zhx1 and hnRNPA1 drives the cardiac progenitor-specific transcriptional activation during cardiomyocyte differentiation
    Article Snippet: .. Total RNA was extracted from cells with TRIzol reagent (Invitrogen), and 500 ng RNA was reverse-transcribed with PrimeScript RT reagent kit (TaKaRa). qPCR was performed with SYBR green reagent (Bio-Rad) in the MX3000 qPCR system (Agilent). ..

    Article Title: LncCMRR Plays an Important Role in Cardiac Differentiation by Regulating the Purb/Flk1 Axis.
    Article Snippet: As crucial epigenetic regulators, long noncoding RNAs (lncRNAs) play critical functions in development processes and various diseases.. However, the regulatory mechanism of lncRNAs in early heart development is still limited.. In this study, we identified cardiac mesodermrelated lncRNA (LncCMRR).

    Article Title: Differential expression and global analysis of miR156/ SQUAMOSA promoter binding-like proteins (SPL) module in oat
    Article Snippet: .. Optical strip tubes were used to perform qRT-PCR analysis using the Mx3000 qPCR system (Stratagene, USA) using 1 μl diluted cDNA, 10 μM gene-specific primers, and 10 μl Brilliant III Ultra-Fast SYBR® Green QPCR Master Mix (Agilent, USA). ..

    Article Title: Transcriptional Analysis of the Pre-Erythrocytic Stages of the Rodent Malaria Parasite, Plasmodium yoelii
    Article Snippet: .. The Brilliant® II SYBR® Green QRT-PCR AffinityScriptTM Master Mix, 2-Step kit (Stratagene), was used to produce cDNA from 24hrLS, 48hrLS, RAS and wtSPZ total RNA derived aRNA, cDNA was diluted 1∶5 with water and 2 ul diluted cDNA was used in 12 ul reactions qPCR reactions performed on a Mx3000 qPCR system (Stratagene). qPCR conditions were as follows, 94°C for minutes followed by 40 cycles of 94°C secs, 55°C 15 secs, 72°C 30 secs, followed by dissociation curve. ..

    Quantitative RT-PCR:

    Article Title: Interplay of starch debranching enzyme and its inhibitor is mediated by Redox-Activated SPL transcription factor
    Article Snippet: A total of 1 μg of extracted RNA was used in cDNA synthesis with AffinityScript QPCR cDNA Synthesis kit (Agilent technology, Canada). .. The qRT-PCR was carried out in the Mx3000 qPCR system (Stratagene, USA). ..

    Article Title: LncCMRR Plays an Important Role in Cardiac Differentiation by Regulating the Purb/Flk1 Axis.
    Article Snippet: As crucial epigenetic regulators, long noncoding RNAs (lncRNAs) play critical functions in development processes and various diseases.. However, the regulatory mechanism of lncRNAs in early heart development is still limited.. In this study, we identified cardiac mesodermrelated lncRNA (LncCMRR).

    Article Title: Differential expression and global analysis of miR156/ SQUAMOSA promoter binding-like proteins (SPL) module in oat
    Article Snippet: .. Optical strip tubes were used to perform qRT-PCR analysis using the Mx3000 qPCR system (Stratagene, USA) using 1 μl diluted cDNA, 10 μM gene-specific primers, and 10 μl Brilliant III Ultra-Fast SYBR® Green QPCR Master Mix (Agilent, USA). ..

    Stripping Membranes:

    Article Title: Differential expression and global analysis of miR156/ SQUAMOSA promoter binding-like proteins (SPL) module in oat
    Article Snippet: .. Optical strip tubes were used to perform qRT-PCR analysis using the Mx3000 qPCR system (Stratagene, USA) using 1 μl diluted cDNA, 10 μM gene-specific primers, and 10 μl Brilliant III Ultra-Fast SYBR® Green QPCR Master Mix (Agilent, USA). ..

    Amplification:

    Article Title: Microsporidia in Commercially Harvested Marine Fish: A Potential Health Risk for Consumers
    Article Snippet: .. Amplification was performed in an Mx3000 qPCR System (Agilent, Santa Clara, CA, USA) with the following cycling conditions: 50 °C for 2 min, 95 °C for 15 min, and 40 cycles of 95 °C for 10 s, 60 °C for 20 s and 72 °C for 20 s. The dissociation curve begins with a 1 min incubation at 95 °C to melt the DNA and then a 30-s incubation at 55 °C, followed by a ramp up to 95 °C with Allpoints data collection performed during the ramp. ..

    Article Title: Microsporidia in Commercially Harvested Marine Fish: A Potential Health Risk for Consumers.
    Article Snippet: .. Amplification was performed in an Mx3000 qPCR System (Agilent, Santa Clara, CA, USA) with the following cycling conditions: 50 ◦C for 2 min, 95 ◦C for 15 min, and 40 cycles of 95 ◦C for 10 s, 60 ◦C for 20 s and 72 ◦C for 20 s. The dissociation curve begins with a 1 min incubation at 95 ◦C to melt the DNA and then a 30-s incubation at 55 ◦C, followed by a ramp up to 95 ◦C with Allpoints data collection performed during the ramp. ..

    Article Title: Distinct Roles for CBP and p300 on the RA-Mediated Expression of the Meiosis Commitment Gene Stra8 in Mouse Embryonic Stem Cells
    Article Snippet: One microgram of RNA from each sample was reverse-transcribed to cDNA using PrimeScriptTM RT reagent kit (TaKaRa). cDNA was amplified in a 20 μl reaction with the Takara Ex Taq PCR kit (TaKaRa). .. PCR amplification was conducted on an automated Stratagene Mx3000 QPCR system (Stratagene). ..

    Incubation:

    Article Title: Microsporidia in Commercially Harvested Marine Fish: A Potential Health Risk for Consumers
    Article Snippet: .. Amplification was performed in an Mx3000 qPCR System (Agilent, Santa Clara, CA, USA) with the following cycling conditions: 50 °C for 2 min, 95 °C for 15 min, and 40 cycles of 95 °C for 10 s, 60 °C for 20 s and 72 °C for 20 s. The dissociation curve begins with a 1 min incubation at 95 °C to melt the DNA and then a 30-s incubation at 55 °C, followed by a ramp up to 95 °C with Allpoints data collection performed during the ramp. ..

    Article Title: Microsporidia in Commercially Harvested Marine Fish: A Potential Health Risk for Consumers.
    Article Snippet: .. Amplification was performed in an Mx3000 qPCR System (Agilent, Santa Clara, CA, USA) with the following cycling conditions: 50 ◦C for 2 min, 95 ◦C for 15 min, and 40 cycles of 95 ◦C for 10 s, 60 ◦C for 20 s and 72 ◦C for 20 s. The dissociation curve begins with a 1 min incubation at 95 ◦C to melt the DNA and then a 30-s incubation at 55 ◦C, followed by a ramp up to 95 ◦C with Allpoints data collection performed during the ramp. ..

    Polymerase Chain Reaction:

    Article Title: Distinct Roles for CBP and p300 on the RA-Mediated Expression of the Meiosis Commitment Gene Stra8 in Mouse Embryonic Stem Cells
    Article Snippet: One microgram of RNA from each sample was reverse-transcribed to cDNA using PrimeScriptTM RT reagent kit (TaKaRa). cDNA was amplified in a 20 μl reaction with the Takara Ex Taq PCR kit (TaKaRa). .. PCR amplification was conducted on an automated Stratagene Mx3000 QPCR system (Stratagene). ..

    Derivative Assay:

    Article Title: Transcriptional Analysis of the Pre-Erythrocytic Stages of the Rodent Malaria Parasite, Plasmodium yoelii
    Article Snippet: .. The Brilliant® II SYBR® Green QRT-PCR AffinityScriptTM Master Mix, 2-Step kit (Stratagene), was used to produce cDNA from 24hrLS, 48hrLS, RAS and wtSPZ total RNA derived aRNA, cDNA was diluted 1∶5 with water and 2 ul diluted cDNA was used in 12 ul reactions qPCR reactions performed on a Mx3000 qPCR system (Stratagene). qPCR conditions were as follows, 94°C for minutes followed by 40 cycles of 94°C secs, 55°C 15 secs, 72°C 30 secs, followed by dissociation curve. ..



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